Assessment of extended – spectrum beta lactamases (ESBL) gene among gram-negative bacilli bacteria causing community acquired urinary tract infection among females in southwestern Nigeria

ESBL and UTI among females in Babcock Community

  • Tinuade Adesola Ajani Department of Medical Microbiology, Ben Carson School of Medicine/ Babcock University Teaching Hospital. Ilishan-Remo, Ogun State, Nigeria.
  • Charles John Elikwu Department of Medical Microbiology, Ben Carson School of Medicine/ Babcock University Teaching Hospital. Ilishan-Remo, Ogun State, Nigeria.
  • Mustapha Akanji Ajani Department of Pathology, College of Medicine, University of Ibadan and University College Hospital
  • Chinenye Gloria Anaedobe Department of Medical Microbiology and Parasitology, University of Abuja, FCT, Nigeria
  • Timothy A. Olusesan Oluwasola Department of Obstetrics and Gynaecology, University College Hospital, Ibadan and College of Medicine, University of Ibadan, Ibadan. Nigeria
Keywords: Gram negative bacilli, Extended - Spectrum beta lactamases, Urinary tract infections, Babcock University

Abstract

Background: Urinary tract infections (UTI) is commoner in females and mainly caused by Gram Negative bacilli. The emergence of Extended – spectrum beta lactamases (ESBL) has made its treatment difficult especially in the community because of limited therapeutic options. This study was undertaken to determine the prevalence of ESBL-producing Gram negative bacilli causing UTI among females in Babcock University Community; evaluate the associated risk factors and to determine the prevalence of the associated genes among the ESBL isolates.

Methods: A descriptive cross-sectional study of which 200 female participants with clinical UTI were recruited in Babcock University Community. Pretested interviewer–based structured questionnaire was used to obtain the socio-demographic and risk factors. Mid-stream urine was also collected for evaluation of ESBL- producing Gram-negative bacilli.  DNA extraction was done for ESBL positive isolates and SHV, TEM, CTX-M and OXA ESBL genes were detected by PCR. SPSS Version 23.0 was used for data analysis.

Results: The 200-urine samples tested from the participants yielded 33-Gram negative bacilli isolates. Out of these 33 isolates, 17/33(51.5%) were ESBL-producers giving prevalence rate of 8.5% among the participants. The distribution of the ESBL genes among the ESBL isolates was as follows: SHV 12/17(70.6%), TEM 4/17(23.5%), CTX-M 3/17(17.6%) and OXA 5/17(29.4%). No associated risk factors were found.

Conclusion: All the four types of ESBL genes evaluated were present among the participants. Routine screening for ESBL for Community acquired UTI is necessary to curb treatment failure.

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Published
2022-03-28
How to Cite
Ajani, T., Elikwu, C., Ajani, M., Anaedobe, C., & Oluwasola, T. (2022). Assessment of extended – spectrum beta lactamases (ESBL) gene among gram-negative bacilli bacteria causing community acquired urinary tract infection among females in southwestern Nigeria. Medical Journal of Zambia, 48(4), 360 - 367. Retrieved from https://mjz.co.zm/index.php/mjz/article/view/725
Section
Original Articles