Evaluation of Von Willebrand Factor and other Coagulation Homeostasis Profile of Patients with Sickle Cell Anaemia attending a Tertiary Hospital at Enugu, Nigeria

  • Solomon Oloche Onoja Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria
  • Blessing Chukwube Eluke Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria
  • Amos Dangana Department of Medical Laboratory Services, University of Abuja Teaching Hospital, Gwagwalada, Abuja Nigeria
  • Sanusi Musa Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria, Nigeria
  • Idris Nasir Abdullahi Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria, Nigeria
Keywords: Sickle Cell, Coagulopathy, Nigeria, Platelet Factors

Abstract

Background:

Sickle cell anaemia (SCA) is a genetic disorder that has been shown to facilitate certain forms of coagulopathy. However, there is a paucity of data that demonstrated these features in Nigerian Sickle cell anaemic patients.

The objective of study:

This prospective study aims to determine the plasma level of vWF, APTT, PT, platelet count and haematological parameters of SCA patients in steady state in comparison with apparently healthy individuals attending the University of Nigeria Teaching Hospital, Enugu, Nigeria.

Materials and Methods:

A total of 85 participants were enrolled in this study. This comprised of 35 homozygous SCA in steady-state as test subjects (HbSS) and 50 apparently healthy individuals of Hb AA as the control group. Blood samples were collected from all participants; both PT and APTT were analysed using Quick and kaolin methods, respectively., vWF was assayed using Enzyme-Linked Immunosorbent Assay (ELISA). Haematological parameters were analysed through the use of an automated haematology analyser.

Results:

There was significant elevation of mean±SD of vWF, APTT and platelet count among SCA patients (52.53 ± 1.45 iu/dl), (48.40 ± 4.99 s) and (287.68 ± 21.43 x109/l) when compared with the mean value in control group (40.16 ±1.50 iu/dl), (33.88 ± 1.34 s) and (219.67 ± 13.9 x109/l) (p< 0.05). However, there were significant decrease in PCV, haemoglobin concentration and red blood cell count among SCA patients, (24.00 ± 0.82 l/l), (7.89 ± 0.24 g/l) and (2.97 ± 0.8 x 1010/l) when compared with control group (40.95 ± 0.73 l/l), (12.44 ± 0.26 g/l) and (4.81 ± 0.11 x 10/l) (p˂ 0.05) respectively. In addition, significant elevation of mean ±SD MCH and MCHC with mean values of (26.63±0.93 pg) and (33.28 ± 0.52 g/l) among subjects when compared with control groups (25.68± 0.47 pg), (30.30 ± 0.18 g/l) (p˂0.005). However, there were no significant differences in WBC, PT and MCV in both study groups.

Conclusion:

Findings from this study demonstrated prolonged coagulation profile, thrombocytosis and leucocytosis among SCA patients in steady state.

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Author Biographies

Solomon Oloche Onoja, Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Medical Laboratory Scientist

Blessing Chukwube Eluke, Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Lecturer

Amos Dangana, Department of Medical Laboratory Services, University of Abuja Teaching Hospital, Gwagwalada, Abuja Nigeria

Chief Medical Laboratory Scientist

Sanusi Musa, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria, Nigeria

Lecturer

Idris Nasir Abdullahi, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria, Nigeria

Lecturer

Published
2021-02-24
How to Cite
Onoja, S., Eluke, B., Dangana, A., Musa, S., & Abdullahi, I. (2021). Evaluation of Von Willebrand Factor and other Coagulation Homeostasis Profile of Patients with Sickle Cell Anaemia attending a Tertiary Hospital at Enugu, Nigeria. Medical Journal of Zambia, 47(4), 269 - 275. Retrieved from https://mjz.co.zm/index.php/mjz/article/view/715
Section
Original Articles